Magen Biotech aims to provide exceptional biotechnology supplies, and their HiPure Circulating DNA Kit is no exception. This kit offers efficient purification of free-circulating nucleic acids, allowing for accurate clinical in vitro detection. With a focus on excellence and reliability, Magen Biotech delivers outstanding products for the research and medical communities.
Principle of Nucleic Acid Purification Using HiPure Circulating DNA Kit
Magen Biotech’s HiPure Circulating DNA Kit employs silica column purification to isolate and purify circulating nucleic acids. The sample is carefully lysed and digested using lysate and protease, releasing DNA into the lysate. The nucleic acids are then adsorbed on a membrane within an adsorption column, while proteins are removed through filtration. After thorough washing to eliminate impurities, the nucleic acids are eluted using a low-salt buffer.
Advantages of HiPure Circulating DNA Kit for Nucleic Acid Purification
The HiPure Circulating DNA Kit stands out due to its numerous advantages that contribute to the efficient and reliable purification of nucleic acids:
1.High Yield: Leveraging the most optimal process, the HiPure Circulating DNA Kit provides maximum recovery of free DNA fragments (>50bp).
2.High Concentration: This kit ensures high nucleic acid concentration by utilizing low elution volumes.
3.High Purity: The HiPure Circulating DNA Kit incorporates a low alcohol binding method that effectively removes inhibitors and protein contaminants, resulting in nucleic acids of exceptional purity.
4.High Recovery: By employing silica gel column purification, the HiPure Circulating DNA Kit allows for DNA recovery at the picogram level, enabling accurate downstream applications.
Storage and Stability of HiPure Circulating DNA Kit
To maintain the performance of the HiPure Circulating DNA Kit, it is recommended to store Proteinase K and Carrier RNA at 2-8°C upon arrival. Although short-term storage at room temperature (15-25°C) for up to 12 weeks does not affect their performance, long-term storage should be done at the recommended temperature. The remaining components of the kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If storing the entire kit at 2-8°C, buffers should be redissolved before use, and it is crucial to ensure that all buffers are at room temperature during the experiment.
Conclusion
Magen Biotech’s HiPure Circulating DNA Kit offers researchers and medical professionals a reliable, efficient, and high-quality solution for nucleic acid purification. With its optimized process, high yield, concentration, purity, and recovery, this kit not only streamlines the purification process but also ensures accurate clinical in vitro detection. The storage and stability features further enhance the convenience and reliability of this exceptional biotechnology supply, making it an excellent choice for laboratories and healthcare facilities.
